Mouse Interleukin 1 receptor antagonist, IL-1ra ELISA kit
Product Details
| Target Name |
interleukin 1 receptor antagonist |
| Alternative Names |
Il1rn ELISA Kit; Il-1raInterleukin-1 receptor antagonist protein ELISA Kit; IL-1RN ELISA Kit; IL-1ra ELISA Kit; IRAP ELISA Kit; IL1 inhibitor ELISA Kit |
| Abbreviation |
IL1RN |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays):CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
81-88 |
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| 1:2 |
Average % |
92 |
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| Range % |
89-96 |
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| 1:4 |
Average % |
102 |
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| Range % |
98-105 |
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| 1:8 |
Average % |
99 |
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| Range % |
95-104 |
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| Recovery |
| The recovery of mouse IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
87 |
83-90 |
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| EDTA plasma (n=4) |
100 |
95-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.547 |
2.601 |
2.574 |
2.413 |
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| 2000 |
1.721 |
1.709 |
1.715 |
1.554 |
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| 1000 |
1.014 |
1.102 |
1.058 |
0.897 |
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| 500 |
0.616 |
0.628 |
0.622 |
0.461 |
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| 250 |
0.360 |
0.371 |
0.366 |
0.205 |
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| 125 |
0.263 |
0.260 |
0.262 |
0.101 |
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| 62.5 |
0.201 |
0.199 |
0.200 |
0.039 |
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| 0 |
0.162 |
0.160 |
0.161 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
