Mouse interleukin 10,IL-10 ELISA KIT
Product Details
| Description |
The product CSB-E04594m is a ready-to-use microwell, strip plate ELISA kit for quantitatively analyzing the presence of the IL-10 in different biological samples, including serum, plasma, cell culture supernates, and tissue homogenates. This IL-10 ELISA kit specifically recognizes the mouse IL-10 in the sample. It adopts the Sandwich ELISA technique in conjunction with antibody-antigen specific binding and an enzyme colorimetric reaction to indicate the presence of mouse IL-10 and quantitatively measure the concentration of IL-10 in the samples.
IL-10 is an immunosuppressive cytokine that predominantly exerts an anti-inflammatory effect. IL-10 mainly counters the synthesis of inflammatory mediators, particularly in response to TLR signaling. The IL-10/STAT3/SOCS3 signaling is involved in critical cellular processes such as cell growth, apoptosis, and transcription of inflammatory genes.
|
| Target Name |
interleukin 10 |
| Alternative Names |
Il10 ELISA Kit; Il-10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit |
| Abbreviation |
IL10 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
3.12 pg/mL-200 pg/mL |
| Sensitivity |
0.78 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
84 |
|
| Range % |
81-94 |
|
| 1:2 |
Average % |
96 |
|
| Range % |
91-100 |
|
| 1:4 |
Average % |
96 |
|
| Range % |
93-104 |
|
| 1:8 |
Average % |
89 |
|
| Range % |
81-103 |
|
|
| Recovery |
| The recovery of mouse IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
81-98 |
|
| EDTA plasma (n=4) |
94 |
91-100 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 200 |
2.272 |
2.152 |
2.212 |
2.068 |
|
| 100 |
1.794 |
1.760 |
1.777 |
1.633 |
|
| 50 |
1.290 |
1.194 |
1.242 |
1.098 |
|
| 25 |
0.787 |
0.769 |
0.778 |
0.634 |
|
| 12.5 |
0.493 |
0.487 |
0.490 |
0.346 |
|
| 6.25 |
0.336 |
0.329 |
0.333 |
0.189 |
|
| 3.12 |
0.236 |
0.219 |
0.228 |
0.084 |
|
| 0 |
0.149 |
0.139 |
0.144 |
|
|
|
|
| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IL-10 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- Biotin-labeled IL-10 antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
- HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
- HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
- Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
- Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
- TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
|
| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
