Mouse Interleukin 13,IL-13 ELISA Kit
Product Details
| Target Name |
interleukin 13 |
| Alternative Names |
Il13 ELISA Kit; Il-13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit; T-cell activation protein P600 ELISA Kit |
| Abbreviation |
IL13 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
88-100 |
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| 1:2 |
Average % |
92 |
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| Range % |
82-98 |
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| 1:4 |
Average % |
92 |
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| Range % |
85-99 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-99 |
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| Recovery |
| The recovery of mouse IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
90-103 |
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| EDTA plasma (n=4) |
92 |
87-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.906 |
2.846 |
2.876 |
2.704 |
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| 1000 |
2.374 |
2.363 |
2.369 |
2.197 |
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| 500 |
1.595 |
1.620 |
1.608 |
1.436 |
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| 250 |
0.896 |
0.867 |
0.882 |
0.710 |
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| 125 |
0.523 |
0.558 |
0.541 |
0.369 |
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| 62.5 |
0.381 |
0.394 |
0.388 |
0.216 |
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| 31.25 |
0.271 |
0.257 |
0.264 |
0.092 |
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| 0 |
0.176 |
0.167 |
0.172 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
