Mouse Interleukin 15,IL-15 ELISA Kit
Product Details
| Target Name |
interleukin 15 |
| Alternative Names |
Il15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit |
| Abbreviation |
IL15 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
80-100 |
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| 1:2 |
Average % |
98 |
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| Range % |
85-105 |
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| 1:4 |
Average % |
96 |
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| Range % |
92-105 |
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| 1:8 |
Average % |
89 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of mouse IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
89-100 |
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| EDTA plasma (n=4) |
93 |
90-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.800 |
2.805 |
2.803 |
2.652 |
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| 200 |
2.148 |
2.158 |
2.153 |
2.002 |
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| 100 |
1.358 |
1.330 |
1.344 |
1.193 |
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| 50 |
0.843 |
0.788 |
0.816 |
0.665 |
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| 25 |
0.458 |
0.468 |
0.463 |
0.312 |
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| 12.5 |
0.302 |
0.322 |
0.312 |
0.161 |
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| 6.25 |
0.212 |
0.224 |
0.218 |
0.067 |
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| 0 |
0.146 |
0.156 |
0.151 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
