Mouse Interleukin 17,IL-17 ELISA Kit
Product Details
| Target Name |
interleukin 17A |
| Alternative Names |
Il17a ELISA Kit; Ctla8 ELISA Kit; Il17 ELISA Kit; Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit; Cytotoxic T-lymphocyte-associated antigen 8 ELISA Kit; CTLA-8 ELISA Kit |
| Abbreviation |
IL17A |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates, cell culture supernates |
| Detection Range |
47 pg/mL-3000 pg/mL |
| Sensitivity |
11.75 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
89-101 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:4 |
Average % |
88 |
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| Range % |
81-99 |
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| 1:8 |
Average % |
90 |
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| Range % |
81-98 |
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| Recovery |
| The recovery of mouse IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
88-100 |
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| EDTA plasma (n=4) |
94 |
90-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 3000 |
2.807 |
2.909 |
2.858 |
2.690 |
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| 1500 |
2.386 |
2.334 |
2.360 |
2.192 |
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| 750 |
1.568 |
1.603 |
1.586 |
1.418 |
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| 375 |
0.835 |
0.874 |
0.855 |
0.687 |
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| 187.5 |
0.442 |
0.459 |
0.451 |
0.283 |
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| 94 |
0.317 |
0.334 |
0.326 |
0.158 |
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| 47 |
0.254 |
0.243 |
0.249 |
0.081 |
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| 0 |
0.162 |
0.174 |
0.168 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
