Mouse Interleukin 18,IL-18 ELISA Kit
Product Details
| Target Name |
interleukin 18 (interferon-gamma-inducing factor) |
| Alternative Names |
Il18 ELISA Kit; Igif ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit |
| Abbreviation |
IL18 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
1.56 pg/mL-100 pg/mL |
| Sensitivity |
0.39 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-94 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-103 |
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| 1:4 |
Average % |
93 |
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| Range % |
88-99 |
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| 1:8 |
Average % |
94 |
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| Range % |
90-100 |
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| Recovery |
| The recovery of mouse IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
90-100 |
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| EDTA plasma (n=4) |
99 |
94-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.646 |
2.552 |
2.599 |
2.484 |
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| 50 |
2.218 |
2.186 |
2.202 |
2.087 |
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| 25 |
1.874 |
1.804 |
1.839 |
1.724 |
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| 12.5 |
1.332 |
1.329 |
1.331 |
1.216 |
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| 6.25 |
0.925 |
0.901 |
0.913 |
0.798 |
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| 3.12 |
0.527 |
0.499 |
0.513 |
0.398 |
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| 1.56 |
0.261 |
0.278 |
0.270 |
0.155 |
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| 0 |
0.119 |
0.111 |
0.115 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
