Mouse Interleukin 29(IL-29) ELISA Kit
Product Details
| Target Name |
Interleukin 29(IL-29) |
| Alternative Names |
IFNL1, IL-29, interferon, lambda 1|interleukin 29 |
| Abbreviation |
IL29 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
12.5 pg/mL-800 pg/mL |
| Sensitivity |
3.1 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-29 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
|
| Range % |
88-97 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-101 |
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| 1:4 |
Average % |
103 |
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| Range % |
100-106 |
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| 1:8 |
Average % |
89 |
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| Range % |
82-96 |
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| Recovery |
| The recovery of mouse IL-29 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
88-96 |
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| EDTA plasma (n=4) |
103 |
99-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.807 |
2.979 |
2.893 |
2.735 |
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| 400 |
2.064 |
2.235 |
2.150 |
1.992 |
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| 200 |
1.341 |
1.423 |
1.382 |
1.224 |
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| 100 |
0.862 |
0.822 |
0.842 |
0.684 |
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| 50 |
0.514 |
0.521 |
0.518 |
0.360 |
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| 25 |
0.335 |
0.331 |
0.333 |
0.175 |
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| 12.5 |
0.249 |
0.257 |
0.253 |
0.095 |
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| 0 |
0.153 |
0.162 |
0.158 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
