Mouse Interleukin 33(IL-33) ELISA Kit
Product Details
| Target Name |
interleukin 33 |
| Alternative Names |
Il33Interleukin-33 ELISA Kit; IL-33) [Cleaved into: Interleukin-33(102-266); Interleukin-33(109-266)] ELISA Kit |
| Abbreviation |
IL33 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
83-95 |
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| 1:2 |
Average % |
92 |
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| Range % |
87-97 |
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| 1:4 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:8 |
Average % |
94 |
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| Range % |
88-98 |
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| Recovery |
| The recovery of mouse IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
92-105 |
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| EDTA plasma (n=4) |
95 |
93-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.771 |
2.752 |
2.762 |
2.605 |
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| 1000 |
2.121 |
2.076 |
2.099 |
1.942 |
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| 500 |
1.414 |
1.404 |
1.409 |
1.252 |
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| 250 |
0.778 |
0.741 |
0.760 |
0.603 |
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| 125 |
0.471 |
0.436 |
0.454 |
0.297 |
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| 62.5 |
0.322 |
0.305 |
0.314 |
0.157 |
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| 31.25 |
0.254 |
0.237 |
0.246 |
0.089 |
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| 0 |
0.160 |
0.153 |
0.157 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
