Mouse Interleukin 35(IL-35) ELISA Kit
Product Details
| Target Name |
Interleukin 35(IL-35) |
| Abbreviation |
IL-35 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
87-97 |
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| 1:2 |
Average % |
95 |
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| Range % |
89-99 |
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| 1:4 |
Average % |
94 |
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| Range % |
90-100 |
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| 1:8 |
Average % |
93 |
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| Range % |
87-97 |
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| Recovery |
| The recovery of mouse IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
87-98 |
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| EDTA plasma (n=4) |
96 |
91-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.461 |
2.391 |
2.426 |
2.235 |
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| 500 |
2.073 |
1.965 |
2.019 |
1.828 |
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| 250 |
1.669 |
1.577 |
1.623 |
1.432 |
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| 125 |
1.272 |
1.183 |
1.228 |
1.037 |
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| 62.5 |
0.814 |
0.804 |
0.809 |
0.618 |
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| 31.2 |
0.567 |
0.525 |
0.546 |
0.355 |
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| 15.6 |
0.335 |
0.314 |
0.325 |
0.134 |
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| 0 |
0.194 |
0.188 |
0.191 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
