Mouse Interleukin 4,IL-4 ELISA KIT
Product Details
| Target Name |
interleukin 4 |
| Alternative Names |
Il4 ELISA kit; Il-4Interleukin-4 ELISA kit; IL-4 ELISA kit; B-cell IgG differentiation factor ELISA kit; B-cell growth factor 1 ELISA kit; B-cell stimulatory factor 1 ELISA kit; BSF-1 ELISA kit; IGG1 induction factor ELISA kit; Lymphocyte stimulatory factor 1 ELISA kit |
| Abbreviation |
IL4 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates, cell culture supernates |
| Detection Range |
1.56 pg/mL-100 pg/mL |
| Sensitivity |
0.39 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:4 |
Average % |
86 |
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| Range % |
80-90 |
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| 1:8 |
Average % |
89 |
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| Range % |
84-97 |
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| Recovery |
| The recovery of mouse IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
92 |
87-95 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.775 |
2.757 |
2.766 |
2.610 |
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| 50 |
2.546 |
2.434 |
2.490 |
2.334 |
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| 25 |
2.053 |
2.008 |
2.031 |
1.875 |
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| 12.5 |
1.397 |
1.345 |
1.371 |
1.215 |
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| 6.25 |
0.816 |
0.786 |
0.801 |
0.645 |
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| 3.12 |
0.478 |
0.462 |
0.470 |
0.314 |
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| 1.56 |
0.278 |
0.263 |
0.271 |
0.115 |
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| 0 |
0.158 |
0.154 |
0.156 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
