Mouse Interleukin 5,IL-5 ELISA KIT
Product Details
| Target Name |
interleukin 5 (colony-stimulating factor, eosinophil) |
| Alternative Names |
Il5 ELISA Kit; Il-5Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit |
| Abbreviation |
IL5 |
| Uniprot No. |
P04401 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-90 |
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| 1:2 |
Average % |
99 |
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| Range % |
93-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-102 |
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| 1:8 |
Average % |
95 |
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| Range % |
91-101 |
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| Recovery |
| The recovery of mouse IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
86-99 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.336 |
2.252 |
2.294 |
2.203 |
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| 1000 |
1.699 |
1.686 |
1.693 |
1.602 |
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| 500 |
1.247 |
1.148 |
1.198 |
1.107 |
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| 250 |
0.913 |
0.877 |
0.895 |
0.804 |
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| 125 |
0.571 |
0.525 |
0.548 |
0.457 |
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| 62.5 |
0.315 |
0.294 |
0.305 |
0.214 |
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| 31.25 |
0.198 |
0.184 |
0.191 |
0.100 |
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| 0 |
0.092 |
0.090 |
0.091 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
