Mouse Interleukin 6,IL-6 ELISA KIT
Product Details
| Target Name |
interleukin 6 (interferon, beta 2) |
| Alternative Names |
Il6 ELISA Kit; Il-6Interleukin-6 ELISA Kit; IL-6 ELISA Kit; B-cell hybridoma growth factor ELISA Kit; Interleukin HP-1 ELISA Kit |
| Abbreviation |
IL6 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
1.56 pg/mL-100 pg/mL |
| Sensitivity |
0.39 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
87-95 |
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| 1:2 |
Average % |
93 |
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| Range % |
85-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-102 |
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| 1:8 |
Average % |
87 |
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| Range % |
82-92 |
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| Recovery |
| The recovery of mouse IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
85-97 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.820 |
2.789 |
2.805 |
2.623 |
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| 50 |
2.307 |
2.238 |
2.273 |
2.091 |
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| 25 |
1.558 |
1.537 |
1.548 |
1.366 |
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| 12.5 |
0.800 |
0.763 |
0.782 |
0.600 |
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| 6.25 |
0.515 |
0.488 |
0.502 |
0.320 |
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| 3.12 |
0.357 |
0.341 |
0.349 |
0.167 |
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| 1.56 |
0.287 |
0.266 |
0.277 |
0.095 |
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| 0 |
0.184 |
0.180 |
0.182 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
