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Mouse Kidney injury molecule 1,Kim-1 ELISA Kit

Product Details

Target Name hepatitis A virus cellular receptor 1
Alternative Names Havcr1 ELISA Kit; Kim1 ELISA Kit; Tim1 ELISA Kit; Timd1 ELISA Kit; Hepatitis A virus cellular receptor 1 homolog ELISA Kit; HAVcr-1 ELISA Kit; Kidney injury molecule 1 ELISA Kit; KIM-1 ELISA Kit; T cell immunoglobulin and mucin domain-containing protein 1 ELISA Kit; TIMD-1 ELISA Kit; T cell membrane protein 1 ELISA Kit; T-cell immunoglobulin mucin receptor 1 ELISA Kit; TIM-1 ELISA Kit; CD antigen CD365 ELISA Kit
Abbreviation HAVCR1
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, urine
Detection Range 0.9 ng/mL-60 ng/mL
Sensitivity 0.225 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Microbiology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%   
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%   
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 87-99  
1:2 Average % 92  
Range % 88-98  
1:4 Average % 96  
Range % 90-101  
1:8 Average % 95  
Range % 92-99  
Recovery
The recovery of mouse Kim-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-97  
EDTA plasma (n=4) 94 88-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
60 2.356 2.345 2.351 2.183  
30 1.786 1.725 1.756 1.588  
15 1.264 1.236 1.250 1.082  
7.5 0.813 0.801 0.807 0.639  
3.75 0.513 0.489 0.501 0.333  
1.8 0.378 0.363 0.371 0.203  
0.9 0.267 0.251 0.259 0.091  
0 0.170 0.166 0.168    
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days