Mouse Lipopolysaccharides(LPS) ELISA Kit
Product Details
| Description |
The mouse Lipopolysaccharides (LPS) ELISA kit allows for the quantitative detection of the mouse LPS in serum, plasma, and tissue homogenates. It has been validated to detect the content of the mouse LPS in the range of 0.156 ng/mL-10 ng/mL. The minimum detectable value is as low as 0.039 ng/mL. Besides, it also has high precision, good linearity, excellent specificity, and high recovery. This kit contains all reagents necessary for quantitatively measuring LPS levels. In this assay, an anti-mouse LPS antibody has been pre-coated on the microplate wells. Samples and standards are respectively pipetted into wells. A biotin-conjugated LPS antibody is subsequently added to wells. The LPS in the samples or the standards is sandwiched between the antibody pairs. Following a thorough washing, the HRP-avidin conjugate is added to wells. The solution turns blue after the addition of the TMB substrate (HRP catalyzes the enzyme-substrate reaction). LPS contains a lipid and a polysaccharide compo... Read more |
| Target Name |
Lipopolysaccharides(LPS) |
| Abbreviation |
LPS |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
85 |
|
| Range % |
80-92 |
|
| 1:2 |
Average % |
98 |
|
| Range % |
91-105 |
|
| 1:4 |
Average % |
102 |
|
| Range % |
92-110 |
|
| 1:8 |
Average % |
96 |
|
| Range % |
86-98 |
|
|
| Recovery |
| The recovery of mouse LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
89-98 |
|
| EDTA plasma (n=4) |
94 |
90-100 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.424 |
2.522 |
2.473 |
2.406 |
|
| 5 |
2.051 |
1.955 |
2.003 |
1.936 |
|
| 2.5 |
1.345 |
1.255 |
1.300 |
1.233 |
|
| 1.25 |
0.679 |
0.655 |
0.667 |
0.600 |
|
| 0.625 |
0.461 |
0.444 |
0.453 |
0.386 |
|
| 0.312 |
0.257 |
0.239 |
0.248 |
0.181 |
|
| 0.156 |
0.171 |
0.165 |
0.168 |
0.101 |
|
| 0 |
0.069 |
0.065 |
0.067 |
|
|
|
|
| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Mouse LPS antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled LPS antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
- An instruction manual
|
| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
