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Mouse Macrophage-Derived Chemokine,MDC ELISA kit

Product Details

Target Name chemokine (C-C motif) ligand 22
Alternative Names Ccl22 ELISA Kit; Abcd1 ELISA Kit; Scya22C-C motif chemokine 22 ELISA Kit; Activated B and dendritic cell-derived ELISA Kit; CC chemokine ABCD-1 ELISA Kit; Small-inducible cytokine A22 ELISA Kit
Abbreviation CCL22
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell culture supernates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 23.672 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MDC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 89    
Range % 83-92    
1:2 Average % 100    
Range % 92-103    
1:4 Average % 94    
Range % 81-98    
1:8 Average % 99    
Range % 90-103    
Recovery
The recovery of mouse MDC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 99 89-103    
EDTA plasma (n=4) 93 84-98    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
2000 2.888 2.812 2.850 2.683    
1000 2.589 2.513 2.551 2.384    
500 2.110 2.168 2.139 1.972    
250 1.463 1.446 1.455 1.288    
125 0.810 0.827 0.819 0.652    
62.5 0.537 0.554 0.546 0.379    
31.25 0.320 0.311 0.316 0.149    
0 0.169 0.165 0.167      
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days