Mouse Macrophage Inflammatory Protein 2,MIP-2 ELISA kit
Product Details
| Target Name |
chemokine (C-X-C motif) ligand 2 |
| Alternative Names |
Cxcl2 ELISA Kit; Mip-2 ELISA Kit; Mip2 ELISA Kit; Scyb2C-X-C motif chemokine 2 ELISA Kit; Macrophage inflammatory protein 2 ELISA Kit; MIP2 ELISA Kit |
| Abbreviation |
CXCL2 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates, cell culture supernates |
| Detection Range |
12.5 pg/mL-800 pg/mL |
| Sensitivity |
3.12 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
85-97 |
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| 1:2 |
Average % |
98 |
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| Range % |
92-102 |
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| 1:4 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:8 |
Average % |
89 |
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| Range % |
87-92 |
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| Recovery |
| The recovery of mouse MIP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
93 |
89-98 |
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| EDTA plasma (n=4) |
99 |
95-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.820 |
2.851 |
2.836 |
2.676 |
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| 400 |
2.124 |
2.102 |
2.113 |
1.953 |
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| 200 |
1.505 |
1.502 |
1.504 |
1.344 |
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| 100 |
0.833 |
0.899 |
0.866 |
0.706 |
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| 50 |
0.519 |
0.527 |
0.523 |
0.363 |
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| 25 |
0.387 |
0.416 |
0.402 |
0.242 |
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| 12.5 |
0.261 |
0.252 |
0.257 |
0.097 |
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| 0 |
0.158 |
0.161 |
0.160 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
