Mouse matrix metalloproteinase 1,MMP-1 ELISA kit
Product Details
| Target Name |
matrix metalloproteinase 1,MMP-1 |
| Alternative Names |
Mmp1a ELISA Kit; McolAInterstitial collagenase A ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1a ELISA Kit; MMP-1a ELISA Kit; Mcol-A ELISA Kit |
| Abbreviation |
MMP-1 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
47 pg/mL-3000 pg/mL |
| Sensitivity |
11.75 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:20 |
Average % |
91 |
|
| Range % |
86-95 |
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| 1:40 |
Average % |
100 |
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| Range % |
97-103 |
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| 1:80 |
Average % |
91 |
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| Range % |
87-95 |
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| 1:160 |
Average % |
97 |
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| Range % |
93-100 |
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| Recovery |
| The recovery of mouse MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
89-97 |
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| EDTA plasma (n=4) |
96 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 3000 |
2.482 |
2.510 |
2.496 |
2.317 |
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| 1500 |
1.855 |
1.786 |
1.821 |
1.642 |
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| 750 |
1.246 |
1.232 |
1.239 |
1.060 |
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| 375 |
0.748 |
0.774 |
0.761 |
0.582 |
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| 187.5 |
0.495 |
0.456 |
0.476 |
0.297 |
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| 93.75 |
0.322 |
0.339 |
0.331 |
0.152 |
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| 46.88 |
0.253 |
0.242 |
0.248 |
0.069 |
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| 0 |
0.180 |
0.178 |
0.179 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
