Mouse matrix metalloproteinase 3,MMP-3 ELISA kit
Product Details
| Target Name |
matrix metallopeptidase 3 (stromelysin 1, progelatinase) |
| Alternative Names |
Mmp3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; EMS-2 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; Transin-1 ELISA Kit |
| Abbreviation |
MMP3 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.78 ng/mL-50 ng/mL |
| Sensitivity |
0.195 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
83-95 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-102 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:8 |
Average % |
93 |
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| Range % |
87-98 |
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| Recovery |
| The recovery of mouse MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
88-95 |
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| EDTA plasma (n=4) |
101 |
95-108 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 50 |
2.367 |
2.264 |
2.316 |
2.157 |
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| 25 |
1.688 |
1.655 |
1.672 |
1.513 |
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| 12.5 |
0.975 |
0.999 |
0.987 |
0.828 |
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| 6.25 |
0.613 |
0.652 |
0.633 |
0.474 |
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| 3.12 |
0.346 |
0.364 |
0.355 |
0.196 |
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| 1.56 |
0.251 |
0.266 |
0.259 |
0.100 |
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| 0.78 |
0.197 |
0.189 |
0.193 |
0.034 |
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| 0 |
0.156 |
0.162 |
0.159 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
