Mouse monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA kit
Product Details
| Target Name |
chemokine (C-C motif) ligand 2 |
| Alternative Names |
Ccl2 ELISA Kit; Je ELISA Kit; Mcp1 ELISA Kit; Scya2 ELISA Kit; C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Platelet-derived growth factor-inducible protein JE ELISA Kit; Small-inducible cytokine A2 ELISA Kit |
| Abbreviation |
CCL2 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
78 pg/mL-5000 pg/mL |
| Sensitivity |
19.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-94 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-98 |
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| 1:4 |
Average % |
102 |
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| Range % |
96-108 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-98 |
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| Recovery |
| The recovery of mouse MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
88 |
82-94 |
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| EDTA plasma (n=4) |
97 |
92-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 5000 |
1.946 |
1.864 |
1.905 |
1.806 |
|
| 2500 |
1.294 |
1.414 |
1.354 |
1.255 |
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| 1250 |
0.861 |
0.843 |
0.852 |
0.753 |
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| 625 |
0.555 |
0.546 |
0.551 |
0.452 |
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| 312 |
0.347 |
0.356 |
0.352 |
0.253 |
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| 156 |
0.267 |
0.258 |
0.263 |
0.164 |
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| 78 |
0.171 |
0.179 |
0.175 |
0.076 |
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| 0 |
0.099 |
0.098 |
0.099 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
