Mouse monocyte chemotactic protein 2,MCP-2 ELISA kit
Product Details
| Target Name |
chemokine (C-C motif) ligand 8 |
| Alternative Names |
Ccl8 ELISA Kit; Mcp2 ELISA Kit; Scya8C-C motif chemokine 8 ELISA Kit; Monocyte chemoattractant protein 2 ELISA Kit; Monocyte chemotactic protein 2 ELISA Kit; MCP-2 ELISA Kit; Small-inducible cytokine A8 ELISA Kit |
| Abbreviation |
CCL8 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
85-88 |
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| 1:2 |
Average % |
102 |
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| Range % |
98-106 |
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| 1:4 |
Average % |
98 |
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| Range % |
96-101 |
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| 1:8 |
Average % |
92 |
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| Range % |
89-95 |
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| Recovery |
| The recovery of mouse MCP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
88 |
84-92 |
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| EDTA plasma (n=4) |
95 |
92-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.955 |
2.781 |
2.868 |
2.720 |
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| 200 |
2.138 |
2.156 |
2.147 |
1.999 |
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| 100 |
1.445 |
1.532 |
1.489 |
1.341 |
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| 50 |
0.921 |
0.903 |
0.912 |
0.764 |
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| 25 |
0.584 |
0.561 |
0.573 |
0.425 |
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| 12.5 |
0.333 |
0.324 |
0.329 |
0.181 |
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| 6.25 |
0.241 |
0.253 |
0.247 |
0.099 |
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| 0 |
0.142 |
0.153 |
0.148 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
