Mouse Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit
roduct Details
| Target Name |
Monocyte Chemotactic Protein 5 (MCP-5) |
| Alternative Names |
C-C motif chemokine 12 ELISA Kit; Ccl12 ELISA Kit; CCL12_MOUSE ELISA Kit; Macrophage/monocyte Chemotactic Protein ELISA Kit; MCP-1-related chemokine ELISA Kit; MCP-5 ELISA Kit; Monocyte chemoattractant protein 5 ELISA Kit; Monocyte chemotactic protein 5 ELISA Kit; OTTMUSP00000000792 ELISA Kit; Scya12 ELISA Kit; Small inducible cytokine A12 ELISA Kit; Small-inducible cytokine A12 ELISA Kit |
| Abbreviation |
MCP-5 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
16.82 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
82-91 |
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| 1:2 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:4 |
Average % |
107 |
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| Range % |
103-110 |
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| 1:8 |
Average % |
110 |
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| Range % |
104-114 |
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| Recovery |
| The recovery of mouse MCP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
91-99 |
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| EDTA plasma (n=4) |
103 |
98-108 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.615 |
2.634 |
2.625 |
2.499 |
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| 500 |
2.177 |
2.012 |
2.095 |
1.969 |
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| 250 |
1.432 |
1.502 |
1.467 |
1.341 |
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| 125 |
0.842 |
0.839 |
0.841 |
0.715 |
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| 62.5 |
0.531 |
0.529 |
0.530 |
0.404 |
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| 31.2 |
0.334 |
0.326 |
0.330 |
0.204 |
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| 15.6 |
0.246 |
0.251 |
0.249 |
0.123 |
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| 0 |
0.125 |
0.127 |
0.126 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
