Mouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit
Product Details
| Target Name |
myeloperoxidase-antineutrophil cytoplasmic antibody |
| Abbreviation |
MAC Ab |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse myeloperoxidase-antineutrophil cytoplasmic antibody （MPO-ANCA）in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-102 |
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| 1:2 |
Average % |
95 |
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| Range % |
83-101 |
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| 1:4 |
Average % |
96 |
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| Range % |
90-106 |
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| 1:8 |
Average % |
94 |
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| Range % |
82-101 |
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| Recovery |
| The recovery of mouse myeloperoxidase-antineutrophil cytoplasmic antibody（MPO-ANCA）spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
89-98 |
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| EDTA plasma (n=4) |
94 |
91-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 40 |
2.404 |
2.418 |
2.411 |
2.297 |
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| 20 |
1.956 |
1.904 |
1.930 |
1.816 |
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| 10 |
1.352 |
1.377 |
1.365 |
1.251 |
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| 5 |
0.902 |
0.934 |
0.918 |
0.804 |
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| 2.5 |
0.526 |
0.534 |
0.530 |
0.416 |
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| 1.25 |
0.311 |
0.326 |
0.319 |
0.205 |
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| 0.625 |
0.265 |
0.271 |
0.268 |
0.154 |
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| 0 |
0.112 |
0.115 |
0.114 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
