Mouse Neurotrophin 3,NT-3 ELISA kit
Product Details
| Target Name |
Neurotrophin 3,NT-3 |
| Alternative Names |
Ntf3 ELISA Kit; Ntf-3 ELISA Kit; Neurotrophin-3 ELISA Kit; NT-3 ELISA Kit; HDNF ELISA Kit; Nerve growth factor 2 ELISA Kit; NGF-2 ELISA Kit; Neurotrophic factor ELISA Kit |
| Abbreviation |
NT-3 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NT-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:1 |
Average % |
105 |
|
|
| Range % |
87-109 |
|
|
| 1:2 |
Average % |
102 |
|
|
| Range % |
97-106 |
|
|
| 1:4 |
Average % |
97 |
|
|
| Range % |
90-100 |
|
|
| 1:8 |
Average % |
98 |
|
|
| Range % |
91-102 |
|
|
|
| Recovery |
| The recovery of mouse NT-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
111 |
100-116 |
|
|
| EDTA plasma (n=4) |
100 |
90-104 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
|
| 4000 |
2.179 |
2.107 |
2.143 |
1.977 |
|
|
| 2000 |
1.533 |
1.578 |
1.556 |
1.390 |
|
|
| 1000 |
1.041 |
1.073 |
1.057 |
0.891 |
|
|
| 500 |
0.655 |
0.678 |
0.667 |
0.501 |
|
|
| 250 |
0.487 |
0.500 |
0.494 |
0.328 |
|
|
| 125 |
0.401 |
0.415 |
0.408 |
0.242 |
|
|
| 62.5 |
0.278 |
0.284 |
0.281 |
0.115 |
|
|
| 0 |
0.168 |
0.164 |
0.166 |
|
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
