Mouse P-Selectin ELISA kit
Product Details
| Target Name |
selectin P (granule membrane protein 140kDa, antigen CD62) |
| Alternative Names |
Selp ELISA Kit; GrmpP-selectin ELISA Kit; CD62 antigen-like family member P ELISA Kit; Granule membrane protein 140 ELISA Kit; GMP-140 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 3 ELISA Kit; LECAM3 ELISA Kit; Platelet activation dependent granule-external membrane protein ELISA Kit; PADGEM ELISA Kit; CD antigen CD62P ELISA Kit |
| Abbreviation |
SELP |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse P-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
92 |
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| Range % |
84-98 |
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| 1:200 |
Average % |
95 |
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| Range % |
81-101 |
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| 1:400 |
Average % |
97 |
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| Range % |
93-103 |
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| 1:800 |
Average % |
95 |
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| Range % |
89-98 |
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| Recovery |
| The recovery of mouse P-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
92-99 |
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| EDTA plasma (n=4) |
97 |
95-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.768 |
2.710 |
2.739 |
2.568 |
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| 1000 |
2.263 |
2.224 |
2.244 |
2.073 |
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| 500 |
1.576 |
1.557 |
1.567 |
1.396 |
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| 250 |
1.026 |
1.004 |
1.015 |
0.844 |
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| 125 |
0.595 |
0.611 |
0.603 |
0.432 |
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| 62.5 |
0.441 |
0.438 |
0.440 |
0.269 |
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| 31.25 |
0.289 |
0.271 |
0.280 |
0.109 |
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| 0 |
0.169 |
0.172 |
0.171 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
