Mouse platelet-derived growth factor-BB,PDGF-BB ELISA Kit
Product Details
| Target Name |
platelet-derived growth factor-BB,PDGF-BB |
| Abbreviation |
PDGF-BB |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.18 ng/mL-12 ng/mL |
| Sensitivity |
0.047 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
82-88 |
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| 1:2 |
Average % |
94 |
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| Range % |
89-98 |
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| 1:4 |
Average % |
104 |
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| Range % |
97-109 |
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| 1:8 |
Average % |
99 |
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| Range % |
93-105 |
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| Recovery |
| The recovery of mouse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-101 |
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| EDTA plasma (n=4) |
89 |
82-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 12 |
2.389 |
2.501 |
2.445 |
2.281 |
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| 6 |
1.445 |
1.529 |
1.487 |
1.323 |
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| 3 |
0.905 |
0.984 |
0.945 |
0.781 |
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| 1.5 |
0.656 |
0.633 |
0.645 |
0.481 |
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| 0.75 |
0.345 |
0.349 |
0.347 |
0.183 |
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| 0.37 |
0.287 |
0.265 |
0.276 |
0.112 |
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| 0.18 |
0.223 |
0.218 |
0.221 |
0.057 |
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| 0 |
0.159 |
0.169 |
0.164 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
