Mouse pro interleukin 1 beta (pro-IL1B) ELISA Kit
Product Details
| Target Name |
pro interleukin 1 beta (pro-IL1B) |
| Abbreviation |
pro-IL1B |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse pro-IL1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
106 |
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| Range % |
101-113 |
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| 1:2 |
Average % |
87 |
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| Range % |
82-91 |
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| 1:4 |
Average % |
94 |
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| Range % |
90-99 |
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| 1:8 |
Average % |
85 |
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| Range % |
80-90 |
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| Recovery |
| The recovery of mouse pro-IL1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
91-100 |
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| EDTA plasma (n=4) |
91 |
85-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.496 |
2.362 |
2.429 |
2.282 |
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| 200 |
1.951 |
1.841 |
1.896 |
1.749 |
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| 100 |
1.349 |
1.319 |
1.334 |
1.187 |
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| 50 |
0.935 |
0.885 |
0.910 |
0.763 |
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| 25 |
0.603 |
0.627 |
0.615 |
0.468 |
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| 12.5 |
0.367 |
0.374 |
0.371 |
0.224 |
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| 6.25 |
0.228 |
0.236 |
0.232 |
0.085 |
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| 0 |
0.144 |
0.150 |
0.147 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
