Mouse Pulmonary Surfactant-associated protein B (SP-B) ELISA kit
Product Details
| Target Name |
surfactant protein B |
| Alternative Names |
Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit |
| Abbreviation |
SFTPB |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.45 ng/mL-30 ng/mL |
| Sensitivity |
0.11 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
86-97 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-102 |
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| 1:4 |
Average % |
88 |
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| Range % |
85-93 |
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| 1:8 |
Average % |
89 |
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| Range % |
83-94 |
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| Recovery |
| The recovery of mouse SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
91 |
87-96 |
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| EDTA plasma (n=4) |
102 |
96-109 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 30 |
2.176 |
2.206 |
2.191 |
2.031 |
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| 15 |
1.511 |
1.544 |
1.528 |
1.368 |
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| 7.5 |
0.897 |
0.970 |
0.934 |
0.774 |
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| 3.75 |
0.612 |
0.618 |
0.615 |
0.455 |
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| 1.8 |
0.362 |
0.392 |
0.377 |
0.217 |
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| 0.9 |
0.279 |
0.267 |
0.273 |
0.113 |
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| 0.45 |
0.225 |
0.220 |
0.223 |
0.063 |
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| 0 |
0.156 |
0.163 |
0.160 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
