Mouse Regenerating islet-derived protein 3-gamma(REG3G) ELISA kit
Product Details
| Target Name |
regenerating islet-derived 3 gamma |
| Alternative Names |
Reg3g ELISA Kit; Pap3 ELISA Kit; Regenerating islet-derived protein 3-gamma ELISA Kit; REG-3-gamma ELISA Kit; Pancreatitis-associated protein 3 ELISA Kit; Regenerating islet-derived protein III-gamma ELISA Kit; Reg III-gamma) [Cleaved into: Regenerating islet-derived protein 3-gamma 16.5 kDa form; Regenerating islet-derived protein 3-gamma 15 kDa form] ELISA Kit |
| Abbreviation |
REG3G |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8>
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10>
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse REG3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
93 |
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| Range % |
89-106 |
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| 1:200 |
Average % |
92 |
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| Range % |
83-100 |
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| 1:400 |
Average % |
95 |
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| Range % |
89-99 |
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| 1:800 |
Average % |
97 |
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| Range % |
89-103 |
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| Recovery |
| The recovery of mouse REG3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
91-98 |
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| EDTA plasma (n=4) |
97 |
92-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.190 |
2.123 |
2.157 |
1.996 |
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| 500 |
1.793 |
1.762 |
1.778 |
1.617 |
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| 250 |
1.295 |
1.257 |
1.276 |
1.115 |
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| 125 |
0.779 |
0.748 |
0.764 |
0.603 |
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| 62.5 |
0.562 |
0.554 |
0.558 |
0.397 |
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| 31.25 |
0.375 |
0.368 |
0.372 |
0.211 |
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| 15.6 |
0.263 |
0.254 |
0.259 |
0.098 |
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| 0 |
0.157 |
0.165 |
0.161 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
