Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit
Product Details
| Target Name |
terminal complement complex C5b-9,TCC C5b-9 |
| Abbreviation |
TCC C5b9 |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
0.78 ng/mL-50 ng/mL |
| Sensitivity |
0.195 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
88 |
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| Range % |
84-96 |
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| 1:200 |
Average % |
98 |
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| Range % |
91-105 |
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| 1:400 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:800 |
Average % |
92 |
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| Range % |
88-96 |
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| Recovery |
| The recovery of mouse TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
87-97 |
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| EDTA plasma (n=4) |
97 |
92-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 50 |
2.684 |
2.810 |
2.747 |
2.596 |
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| 25 |
2.054 |
2.212 |
2.133 |
1.982 |
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| 12.5 |
1.426 |
1.446 |
1.436 |
1.285 |
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| 6.25 |
0.854 |
0.867 |
0.861 |
0.710 |
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| 3.12 |
0.622 |
0.585 |
0.604 |
0.453 |
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| 1.56 |
0.337 |
0.350 |
0.344 |
0.193 |
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| 0.78 |
0.258 |
0.264 |
0.261 |
0.110 |
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| 0 |
0.148 |
0.154 |
0.151 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
