The Mouse thyroxine (T4) ELISA Kit is designed for the quantitative determination of endogenic mouse T4 concentrations in serum, plasma, or tissue homogenates. This assay employs the competitive inhibition enzyme immunoassay technique. Standards or samples are added to the appropriate anti-mouse T4 antibody-pre-coated microtiter plate wells with a Biotin-conjugated T4 antibody. The competitive inhibition reaction is launched between Biotin-labeled T4 and T4 in the sample or standard with the immobilized T4 antibody. Substrate A and B solution are mixed and then added to the wells, and the color turns blue. The color development is terminated after the addition of a stop solution, and the intensity of the color is opposite to the amount of T4 in the sample. Read the OD value with the Microplate reader and calculated the T4 concentration referring to the standard curve. This kit has been quality tested by multiple criteria, including sensitivity, specificity, precision, linearity, and lo...Read more