Mouse Vascular Endothelial cell Growth Factor C,VEGF-C ELISA kit
Product Details
| Target Name |
vascular endothelial growth factor C |
| Alternative Names |
VegfcVascular endothelial growth factor C ELISA kit; VEGF-C ELISA kit; Flt4 ligand ELISA kit; Flt4-L ELISA kit; Vascular endothelial growth factor-related protein ELISA kit; VRP ELISA kit |
| Abbreviation |
VEGFC |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates, cell lysates |
| Detection Range |
1.56 pg/mL-100 pg/mL |
| Sensitivity |
0.39 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
102 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
90 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of mouse VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-98 |
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| EDTA plasma (n=4) |
96 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.600 |
2.511 |
2.556 |
2.462 |
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| 50 |
2.020 |
2.152 |
2.086 |
1.992 |
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| 25 |
1.391 |
1.366 |
1.379 |
1.285 |
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| 12.5 |
0.735 |
0.744 |
0.740 |
0.646 |
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| 6.25 |
0.331 |
0.321 |
0.326 |
0.232 |
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| 3.12 |
0.255 |
0.235 |
0.245 |
0.151 |
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| 1.56 |
0.188 |
0.186 |
0.187 |
0.093 |
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| 0 |
0.097 |
0.091 |
0.094 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
