Mouse Vascular Endothelial cell Growth Factor D,VEGF-D ELISA kit
Product Details
| Target Name |
c-fos induced growth factor (vascular endothelial growth factor D) |
| Alternative Names |
Vegfd ELISA Kit; Figf ELISA Kit; Vascular endothelial growth factor D ELISA Kit; VEGF-D ELISA Kit; c-Fos-induced growth factor ELISA Kit; FIGF ELISA Kit |
| Abbreviation |
Vegfd |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
0.312 pg/mL-20 pg/mL |
| Sensitivity |
0.078 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:100 |
Average % |
95 |
|
| Range % |
90-99 |
|
| 1:200 |
Average % |
90 |
|
| Range % |
85-96 |
|
| 1:400 |
Average % |
96 |
|
| Range % |
82-105 |
|
| 1:800 |
Average % |
91 |
|
| Range % |
85-99 |
|
|
| Recovery |
| The recovery of mouse VEGF-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
88-94 |
|
| EDTA plasma (n=4) |
95 |
89-100 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20 |
2.857 |
2.705 |
2.781 |
2.647 |
|
| 10 |
2.312 |
2.239 |
2.276 |
2.142 |
|
| 5 |
1.594 |
1.531 |
1.563 |
1.429 |
|
| 2.5 |
0.873 |
0.844 |
0.859 |
0.725 |
|
| 1.25 |
0.550 |
0.568 |
0.559 |
0.425 |
|
| 0.625 |
0.341 |
0.344 |
0.343 |
0.209 |
|
| 0.312 |
0.258 |
0.249 |
0.254 |
0.120 |
|
| 0 |
0.133 |
0.135 |
0.134 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
