Pig apolipoprotein E (Apo-E) ELISA Kit
Product Details
| Target Name |
apolipoprotein E (Apo-E) |
| Alternative Names |
APOEApolipoprotein E ELISA kit; Apo-E ELISA kit |
| Abbreviation |
APOE |
| Species |
Sus scrofa (Pig) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 ng/mL-2000 ng/mL |
| Sensitivity |
7.81 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:100 |
Average % |
96 |
|
|
| Range % |
85-105 |
|
|
| 1:200 |
Average % |
90 |
|
|
| Range % |
89-98 |
|
|
| 1:400 |
Average % |
96 |
|
|
| Range % |
82-103 |
|
|
| 1:800 |
Average % |
97 |
|
|
| Range % |
92-99 |
|
|
|
| Recovery |
| The recovery of pig Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
97 |
92-105 |
|
|
| EDTA plasma (n=4) |
95 |
85-100 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| ng/ml |
OD1 |
OD2 |
Average |
|
|
|
| 2000 |
0.131 |
0.134 |
0.133 |
|
|
|
| 1000 |
0.223 |
0.226 |
0.225 |
|
|
|
| 500 |
0.335 |
0.341 |
0.338 |
|
|
|
| 250 |
0.562 |
0.553 |
0.558 |
|
|
|
| 125 |
0.862 |
0.860 |
0.861 |
|
|
|
| 62.5 |
1.192 |
1.214 |
1.203 |
|
|
|
| 31.25 |
1.589 |
1.607 |
1.598 |
|
|
|
| 0 |
2.417 |
2.395 |
2.406 |
|
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
