Pig Cystatin C,Cys-C ELISA Kit
Product Details
| Target Name |
Cystatin C,Cys-C |
| Abbreviation |
CST3 |
| Species |
Sus scrofa (Pig) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.78 μg/mL-50 μg/mL |
| Sensitivity |
0.2 μg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of pig Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
82-92 |
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| 1:2 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:4 |
Average % |
92 |
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| Range % |
84-96 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-100 |
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| Recovery |
| The recovery of pig Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
9-103 |
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| EDTA plasma (n=4) |
93 |
89-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
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| 50 |
0.202 |
0.205 |
0.204 |
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| 25 |
0.305 |
0.307 |
0.306 |
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| 12.5 |
0.446 |
0.431 |
0.439 |
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| 6.25 |
0.646 |
0.658 |
0.652 |
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| 3.12 |
0.851 |
0.863 |
0.857 |
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| 1.56 |
1.582 |
1.497 |
1.540 |
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| 0.78 |
2.045 |
1.973 |
2.009 |
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| 0 |
2.758 |
2.604 |
2.681 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
