Pig insulin-like growth factors-1,IGF-1 ELISA Kit
Product Details
| Target Name |
insulin-like growth factor 1 (somatomedin C) |
| Alternative Names |
IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit |
| Abbreviation |
IGF1 |
| Species |
Sus scrofa (Pig) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates, cell lysates |
| Detection Range |
6.25 ng/mL-400 ng/mL |
| Sensitivity |
1.56 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-98 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-104 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
95 |
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| Range % |
82-100 |
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| Recovery |
| The recovery of pig IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
85-108 |
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| EDTA plasma (n=4) |
95 |
91-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.824 |
2.815 |
2.820 |
2.676 |
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| 200 |
2.362 |
2.346 |
2.354 |
2.210 |
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| 100 |
1.469 |
1.489 |
1.479 |
1.335 |
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| 50 |
0.709 |
0.684 |
0.697 |
0.553 |
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| 25 |
0.416 |
0.425 |
0.421 |
0.277 |
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| 12.5 |
0.251 |
0.262 |
0.257 |
0.113 |
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| 6.25 |
0.199 |
0.192 |
0.196 |
0.052 |
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| 0 |
0.146 |
0.142 |
0.144 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
