Pig interleukin 10,IL-10 ELISA Kit
Product Details
| Target Name |
interleukin 10 |
| Alternative Names |
IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit |
| Abbreviation |
IL10 |
| Species |
Sus scrofa (Pig) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
81-103 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-105 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-108 |
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| 1:8 |
Average % |
96 |
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| Range % |
82-112 |
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| Recovery |
| The recovery of pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
85-109 |
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| EDTA plasma (n=4) |
97 |
92-106 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.178 |
2.152 |
2.165 |
2.015 |
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| 200 |
1.584 |
1.560 |
1.572 |
1.422 |
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| 100 |
0.981 |
0.950 |
0.966 |
0.816 |
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| 50 |
0.568 |
0.564 |
0.566 |
0.416 |
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| 25 |
0.372 |
0.361 |
0.367 |
0.217 |
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| 12.5 |
0.296 |
0.302 |
0.299 |
0.149 |
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| 6.25 |
0.252 |
0.248 |
0.250 |
0.100 |
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| 0 |
0.152 |
0.148 |
0.150 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
