Pig Interleukin 15(IL-15) ELISA Kit
Product Details
| Target Name |
interleukin 15 |
| Alternative Names |
IL15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit |
| Abbreviation |
IL15 |
| Species |
Sus scrofa (Pig) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates, cell lysates |
| Detection Range |
0.78 pg/mL-50 pg/mL |
| Sensitivity |
0.195 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-89 |
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| 1:2 |
Average % |
102 |
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| Range % |
97-107 |
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| 1:4 |
Average % |
87 |
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| Range % |
84-91 |
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| 1:8 |
Average % |
84 |
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| Range % |
81-89 |
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| Recovery |
| The recovery of pig IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
100 |
97-103 |
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| EDTA plasma (n=4) |
84 |
80-88 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.602 |
2.576 |
2.589 |
2.400 |
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| 25 |
1.879 |
1.850 |
1.865 |
1.676 |
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| 12.5 |
1.144 |
1.168 |
1.156 |
0.967 |
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| 6.25 |
0.683 |
0.691 |
0.687 |
0.498 |
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| 3.12 |
0.452 |
0.468 |
0.460 |
0.271 |
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| 1.56 |
0.361 |
0.391 |
0.376 |
0.187 |
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| 0.78 |
0.288 |
0.298 |
0.293 |
0.104 |
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| 0 |
0.191 |
0.186 |
0.189 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
