Pig interleukin 4,IL-4 ELISA Kit
Product Details
| Target Name |
interleukin 4 |
| Alternative Names |
IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit |
| Abbreviation |
IL4 |
| Species |
Sus scrofa (Pig) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
82-91 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-95 |
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| 1:4 |
Average % |
89 |
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| Range % |
84-93 |
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| 1:8 |
Average % |
96 |
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| Range % |
92-101 |
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| Recovery |
| The recovery of pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
100 |
95-105 |
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| EDTA plasma (n=4) |
92 |
86-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.719 |
2.652 |
2.686 |
2.492 |
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| 200 |
1.962 |
1.861 |
1.912 |
1.718 |
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| 100 |
1.213 |
1.204 |
1.209 |
1.015 |
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| 50 |
0.748 |
0.689 |
0.719 |
0.525 |
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| 25 |
0.551 |
0.527 |
0.539 |
0.345 |
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| 12.5 |
0.355 |
0.374 |
0.365 |
0.171 |
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| 6.25 |
0.295 |
0.272 |
0.284 |
0.090 |
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| 0 |
0.192 |
0.196 |
0.194 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
