Pig Interleukin 6,IL-6 ELISA Kit
Product Details
| Target Name |
interleukin 6 (interferon, beta 2) |
| Alternative Names |
IL6 ELISA Kit; Interleukin-6 ELISA Kit; IL-6 ELISA Kit |
| Abbreviation |
IL6 |
| Species |
Sus scrofa (Pig) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.25 pg/mL-80 pg/mL |
| Sensitivity |
0.31 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
80-90 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-100 |
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| 1:4 |
Average % |
97 |
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| Range % |
95-103 |
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| 1:8 |
Average % |
90 |
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| Range % |
85-95 |
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| Recovery |
| The recovery of pig IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
85-95 |
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| EDTA plasma (n=4) |
99 |
95-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 80 |
2.212 |
2.101 |
2.157 |
2.066 |
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| 40 |
1.725 |
1.704 |
1.715 |
1.624 |
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| 20 |
1.223 |
1.194 |
1.209 |
1.118 |
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| 10 |
0.871 |
0.809 |
0.840 |
0.749 |
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| 5 |
0.519 |
0.504 |
0.512 |
0.421 |
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| 2.5 |
0.332 |
0.315 |
0.324 |
0.233 |
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| 1.25 |
0.192 |
0.182 |
0.187 |
0.096 |
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| 0 |
0.093 |
0.089 |
0.091 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
