Pig major acute phase protein (MAP) ELISA Kit
Product Details
| Target Name |
inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein) |
| Alternative Names |
ITIH4 ELISA Kit; IHRP ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; ITI heavy chain H4 ELISA Kit; ITI-HC4 ELISA Kit; Inter-alpha-inhibitor heavy chain 4 ELISA Kit; Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit; IHRP ELISA Kit; Major acute phase protein ELISA Kit; MAP ELISA Kit |
| Abbreviation |
ITIH4 |
| Species |
Sus scrofa (Pig) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
37.5 ng/mL-2400 ng/mL |
| Sensitivity |
9.38 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of pig MAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
97 |
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| Range % |
92-104 |
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| 1:400 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:800 |
Average % |
93 |
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| Range % |
87-99 |
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| 1:1600 |
Average % |
86 |
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| Range % |
82-92 |
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| Recovery |
| The recovery of pig MAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-101 |
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| EDTA plasma (n=4) |
97 |
90-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 2400 |
0.138 |
0.141 |
0.140 |
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| 1200 |
0.457 |
0.460 |
0.459 |
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| 600 |
0.903 |
0.898 |
0.901 |
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| 300 |
1.134 |
1.121 |
1.128 |
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| 150 |
1.518 |
1.483 |
1.501 |
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| 75 |
1.689 |
1.706 |
1.698 |
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| 37.5 |
2.032 |
2.049 |
2.041 |
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| 0 |
2.187 |
2.114 |
2.151 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
