Pig Mucin-2,MUC2 ELISA Kit
Product Details
| Target Name |
Mucin-2,MUC2 |
| Abbreviation |
MUC2 |
| Species |
Sus scrofa (Pig) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
7.8 ng/mL-500 ng/mL |
| Sensitivity |
1.95 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of pig mucin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
91 |
|
| Range % |
87-94 |
|
| 1:2 |
Average % |
97 |
|
| Range % |
92-101 |
|
| 1:4 |
Average % |
90 |
|
| Range % |
87-98 |
|
| 1:8 |
Average % |
92 |
|
| Range % |
85-95 |
|
|
| Recovery |
| The recovery of pig mucin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
93 |
88-96 |
|
| EDTA plasma (n=4) |
98 |
95-102 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 500 |
1.976 |
2.121 |
2.049 |
1.885 |
|
| 250 |
1.303 |
1.210 |
1.257 |
1.093 |
|
| 125 |
0.774 |
0.784 |
0.779 |
0.615 |
|
| 62.5 |
0.428 |
0.425 |
0.427 |
0.263 |
|
| 31.25 |
0.296 |
0.274 |
0.285 |
0.121 |
|
| 15.62 |
0.235 |
0.250 |
0.243 |
0.079 |
|
| 7.8 |
0.208 |
0.207 |
0.208 |
0.044 |
|
| 0 |
0.158 |
0.169 |
0.164 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
