Pig Stromal cell derived factor 1a,SDF-1a ELISA Kit
Product Details
| Target Name |
Stromal cell derived factor 1a,SDF-1a |
| Abbreviation |
SDF1a/CXCL12 |
| Species |
Sus scrofa (Pig) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates, cell Lysates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of pig SDF-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:2 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:4 |
Average % |
86 |
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| Range % |
82-90 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-101 |
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| Recovery |
| The recovery of pig SDF-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
98 |
94-102 |
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| EDTA plasma (n=4) |
86 |
82-90 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.812 |
2.701 |
2.757 |
2.564 |
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| 10 |
2.397 |
2.361 |
2.379 |
2.186 |
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| 5 |
1.798 |
1.712 |
1.755 |
1.562 |
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| 2.5 |
1.011 |
0.979 |
0.995 |
0.802 |
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| 1.25 |
0.607 |
0.634 |
0.621 |
0.428 |
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| 0.625 |
0.403 |
0.427 |
0.415 |
0.222 |
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| 0.312 |
0.304 |
0.321 |
0.313 |
0.120 |
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| 0 |
0.188 |
0.198 |
0.193 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
