Pig Vascular cell adhesion molecule 1,VCAM-1 ELISA Kit
Product Details
| Target Name |
Vascular cell adhesion molecule 1,VCAM-1 |
| Abbreviation |
VCAM1 |
| Species |
Sus scrofa (Pig) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
15.6ng/mL-1000ng/mL |
| Sensitivity |
3.9 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of pig VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
85-94 |
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| 1:2 |
Average % |
94 |
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| Range % |
87-98 |
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| 1:4 |
Average % |
103 |
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| Range % |
97-107 |
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| 1:8 |
Average % |
101 |
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| Range % |
95-104 |
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| Recovery |
| The recovery of pig VCAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
89 |
83-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.335 |
2.178 |
2.257 |
2.100 |
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| 500 |
1.574 |
1.475 |
1.525 |
1.368 |
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| 250 |
1.007 |
0.982 |
0.995 |
0.838 |
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| 125 |
0.591 |
0.586 |
0.589 |
0.432 |
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| 62.5 |
0.386 |
0.394 |
0.390 |
0.233 |
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| 31.2 |
0.278 |
0.282 |
0.280 |
0.123 |
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| 15.6 |
0.228 |
0.225 |
0.227 |
0.070 |
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| 0 |
0.158 |
0.156 |
0.157 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
