Plant Ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBisCO) ELISA Kit
Product Details
| Target Name |
Ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBisCO) |
| Abbreviation |
RuBisCO |
| Species |
Plant (Mus musculus (Mouse)-Ear Cress) |
| Sample Types |
plant tissues |
| Detection Range |
3.12 μg/mL-800 μg/mL |
| Sensitivity |
3.12 μg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Recovery |
| The recovery of plant RuBisCO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| leaf tissue (n=5) |
95 |
89-103 |
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
|
|
| 800 |
0.047 |
0.050 |
0.049 |
|
|
| 200 |
0.261 |
0.268 |
0.265 |
|
|
| 50 |
0.467 |
0.473 |
0.470 |
|
|
| 12.5 |
0.670 |
0.682 |
0.676 |
|
|
| 3.12 |
1.139 |
1.143 |
1.141 |
|
|
| 0 |
1.861 |
1.916 |
1.889 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
