Rabbit apolipoprotein A1 (Apo-A1) ELISA Kit
Product Details
| Target Name |
apolipoprotein A-I |
| Alternative Names |
APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit |
| Abbreviation |
APOA1 |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 μg/mL-400 μg/mL |
| Sensitivity |
1.56 μg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
99 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
89 |
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| Range % |
80-96 |
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| 1:8 |
Average % |
94 |
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| Range % |
85-99 |
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| Recovery |
| The recovery of rabbit Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
93 |
89-98 |
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| EDTA plasma (n=4) |
98 |
89-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| µg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.748 |
2.712 |
2.730 |
2.645 |
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| 200 |
2.234 |
2.222 |
2.228 |
2.143 |
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| 100 |
1.517 |
1.446 |
1.482 |
1.397 |
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| 50 |
0.875 |
0.839 |
0.857 |
0.772 |
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| 25 |
0.433 |
0.424 |
0.429 |
0.344 |
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| 12.5 |
0.212 |
0.206 |
0.209 |
0.124 |
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| 6.25 |
0.141 |
0.138 |
0.140 |
0.055 |
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| 0 |
0.087 |
0.083 |
0.085 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
