Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit
Product Details
| Target Name |
apolipoprotein B100 (Apo-B100) |
| Alternative Names |
APOBApolipoprotein B ELISA kit; Fragment ELISA kit |
| Abbreviation |
Apo-B100 |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
78 ng/mL-5000 ng/mL |
| Sensitivity |
19.5 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8>
| |
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10>
| |
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:100 |
Average % |
96 |
|
| Range % |
90-102 |
|
| 1:200 |
Average % |
92 |
|
| Range % |
87-96 |
|
| 1:400 |
Average % |
104 |
|
| Range % |
98-108 |
|
| 1:800 |
Average % |
85 |
|
| Range % |
80-89 |
|
|
| Recovery |
| The recovery of rabbit Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
98 |
93-102 |
|
| EDTA plasma (n=4) |
87 |
82-92 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
|
|
| 5000 |
0.092 |
0.091 |
0.092 |
|
|
| 2500 |
0.146 |
0.138 |
0.142 |
|
|
| 1250 |
0.246 |
0.236 |
0.241 |
|
|
| 625 |
0.375 |
0.360 |
0.368 |
|
|
| 312 |
0.524 |
0.539 |
0.532 |
|
|
| 156 |
0.934 |
0.877 |
0.906 |
|
|
| 78 |
1.447 |
1.334 |
1.391 |
|
|
| 0 |
2.432 |
2.562 |
2.497 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
