Rabbit C-Peptide ELISA kit
Product Details
| Target Name |
C-Peptide |
| Abbreviation |
C-Peptide |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma |
| Detection Range |
0.5 ng/mL-15 ng/mL |
| Sensitivity |
0.3 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:4 |
Average % |
98 |
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| Range % |
93-104 |
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| 1:8 |
Average % |
87 |
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| Range % |
81-92 |
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| Recovery |
| The recovery of rabbit C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-97 |
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| EDTA plasma (n=4) |
97 |
92-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 15 |
1.954 |
1.905 |
1.930 |
1.895 |
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| 8 |
1.432 |
1.358 |
1.395 |
1.360 |
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| 4 |
0.782 |
0.791 |
0.787 |
0.752 |
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| 2 |
0.414 |
0.428 |
0.421 |
0.386 |
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| 0.5 |
0.168 |
0.164 |
0.166 |
0.131 |
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| 0 |
0.034 |
0.035 |
0.035 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
