Rabbit Endothelin 1,ET-1 ELISA Kit
Product Details
| Target Name |
endothelin 1 |
| Alternative Names |
EDN1Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit |
| Abbreviation |
EDN1 |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
100 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
96 |
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| Range % |
92-104 |
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| 1:8 |
Average % |
94 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of rabbit ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-99 |
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| EDTA plasma (n=4) |
98 |
90-106 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.630 |
2.456 |
2.543 |
2.458 |
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| 200 |
2.111 |
2.076 |
2.094 |
2.009 |
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| 100 |
1.459 |
1.443 |
1.451 |
1.366 |
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| 50 |
0.816 |
0.769 |
0.793 |
0.708 |
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| 25 |
0.415 |
0.389 |
0.402 |
0.317 |
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| 12.5 |
0.218 |
0.203 |
0.211 |
0.126 |
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| 6.25 |
0.147 |
0.149 |
0.148 |
0.063 |
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| 0 |
0.088 |
0.082 |
0.085 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
