Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit
Product Details
| Target Name |
insulin-like growth factor 1 (somatomedin C) |
| Alternative Names |
IGF1 ELISA Kit; IGF-1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit |
| Abbreviation |
IGF1 |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
80-100 |
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| 1:2 |
Average % |
100 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
98 |
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| Range % |
92-115 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of rabbit IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-98 |
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| EDTA plasma (n=4) |
90 |
85-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.715 |
2.725 |
2.720 |
2.605 |
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| 5 |
2.035 |
2.002 |
2.019 |
1.904 |
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| 2.5 |
0.989 |
1.078 |
1.034 |
0.919 |
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| 1.25 |
0.591 |
0.602 |
0.597 |
0.482 |
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| 0.625 |
0.342 |
0.352 |
0.347 |
0.232 |
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| 0.312 |
0.201 |
0.212 |
0.207 |
0.092 |
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| 0.156 |
0.175 |
0.185 |
0.180 |
0.065 |
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| 0 |
0.111 |
0.119 |
0.115 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
