Rabbit Interleukin 10,IL-10 ELISA Kit
Product Details
| Target Name |
interleukin 10 |
| Alternative Names |
IL10 ELISA Kit; Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit |
| Abbreviation |
IL10 |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
80-98 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-100 |
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| Recovery |
| The recovery of rabbit IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
89 |
80-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.627 |
2.549 |
2.588 |
2.461 |
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| 1000 |
1.954 |
1.799 |
1.877 |
1.750 |
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| 500 |
1.004 |
0.987 |
0.996 |
0.869 |
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| 250 |
0.599 |
0.554 |
0.577 |
0.450 |
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| 125 |
0.369 |
0.361 |
0.365 |
0.238 |
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| 62.5 |
0.274 |
0.264 |
0.269 |
0.142 |
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| 31.25 |
0.177 |
0.171 |
0.174 |
0.047 |
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| 0 |
0.132 |
0.122 |
0.127 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
